HPSI0414i-zazi_5
WTSIi422-B
General#
Cell Line |
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hPSCreg Name | WTSIi422-B |
Alternative name(s) |
HPSI0414i-zazi_5
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 14th May 2018 |
User feedback | |
Provider |
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Generator | Wellcome Trust Sanger Institute (WTSI) |
Distributors | |
External Databases |
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BioSamples | SAMEA3976801 |
ECACC | 77650319 |
HipSci | HPSI0414i-zazi_5 |
Cellosaurus | CVCL_AX02 |
General Information |
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Projects | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA2482384 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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Age of donor (at collection) | 25-29 |
Collected in | 2015 |
Source cell line vendor | University College London |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Notes on reprogramming vector detection | CytoTune 2 |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unkown |
Derived under GMP? |
Unkown |
Available as clinical grade? |
Unkown |
Culture Conditions#
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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CO2 Concentration | 5 % |
Medium |
TeSR™ E8™
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Characterisation#
Analysis of Undifferentiated Cells
Pluripotency Score | Novelty Score | |
24.599 | 1.298 |
Report
HPSI-zazi.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-zazi.pluritest.novelty_score.20161010.png
novelty image
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-zazi_5 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145 Text file with probe intensities |
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