HPSI0414i-zazi_5
WTSIi422-B
General#
Cell Line |
|
| hPSCreg Name | WTSIi422-B |
| Alternative name(s) |
HPSI0414i-zazi_5
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 14th May 2018 |
| User feedback | |
Provider |
|
| Generator | Wellcome Trust Sanger Institute (WTSI) |
| Distributors | |
External Databases |
|
| BioSamples | SAMEA3976801 |
| ECACC | 77650319 |
| HipSci | HPSI0414i-zazi_5 |
| Cellosaurus | CVCL_AX02 |
General Information |
|
| Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Donor Relations |
|
| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA2482384 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
|
| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
|
| Age of donor (at collection) | 25-29 |
| Collected in | 2015 |
| Source cell line vendor | University College London |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Notes on reprogramming vector detection | CytoTune 2 |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Pluripotency Score | Novelty Score | |
| 24.599 | 1.298 |
Report
HPSI-zazi.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-zazi.pluritest.novelty_score.20161010.png
novelty image
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
|
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
No
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0414i-zazi_5 Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Methylation profiling
https://ega-archive.org/datasets/EGAD00010001145 Text file with probe intensities |
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