General

Cell Line
hPSCreg name	UPSFRi005-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	UPSFRi005-A-1 (RRID:CVCL_C350)
Alternative name(s)	PB06-1
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	UPSFRi005-A (PB06) Donor diseases: Down syndrome NCHi004-A (NCH140) Donor diseases: Down Syndrome SCVIi085-A Donor diseases: Down syndrome SDQLCHi065-A Donor diseases: Down syndrome SCVIi086-A Donor diseases: Down syndrome WISCi005-A (DS1, UWWC1-DS1) Donor diseases: Trisomy 21 WISCi006-A (2DS3, UWWC1-2DS3) Donor diseases: Down syndrome WISCi005-B (DS4, UWWC1-DS4) Donor diseases: Trisomy 21 WISCi005-C (UWWC1-DS2U, DS2U) Donor diseases: Trisomy 21 NCHi010-A (NCH141) Donor diseases: Down syndrome NSi003-A (NSi-DS) Donor diseases: Down Syndrome NSi003-B (NSi-isoEu) Donor diseases: Down Syndrome
Last update	27th October 2017
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Provider
Generator	Université Paris-Sud 11 (UPSFR) Contact: ESTeam Paris Sud
Derivation country	France
External Databases
Cellosaurus	CVCL_C350
Wikidata	Q54991772
General Information
* Is the cell line readily obtainable for third parties?	Yes
Subclone of	UPSFRi005-A

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Down syndrome The donor is affected. Synonyms DOWN'S SYNDROME Trisomy 21 NOS Trisomy 21, Mitotic Nondisjunction Trisomy 21, Meiotic Nondisjunction Down's syndrome NOS (disorder) Complete trisomy 21 syndrome (disorder) G Trisomy Down's syndrome NOS Partial Trisomy 21 Down Syndrome Down Syndrome, Partial Trisomy 21 Syndrome, Down's T21 - Trisomy 21 Syndrome, Down trisomy 21 syndrome Mongolism Downs Syndrome Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome Down's syndrome - trisomy 21 Downs syndrome trisomy 21 (Down syndrome) Down's syndrome G trisomy Down syndrome Complete trisomy 21 syndrome Down syndrome, Isolated cases leukemia, megakaryoblastic, with or without Down syndrome, somatic Trisomy 21 complete trisomy 21 syndrome trisomy 21 Down syndrome chromosome region Down syndrome critical region leukemia, megakaryoblastic, of Down syndrome transient myeloproliferative disorder of Down syndrome show more synonyms show less synonyms

Ethics

Also have a look at the ethics information for the parental line UPSFRi005-A .

Alternatives to consent
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General
The source cell information can be found in the parental cell line UPSFRi005-A.
Reprogramming method
Vector type	Integrating
Vector	Virus (Lentivirus)
Genes	POU5F1 SOX2 LIN28 NANOG
Is the used vector excisable?	Unknown
Absence of reprogramming vector(s)?	Unknown
Reprogramming vectors silenced?
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
CO2 Concentration	5 %
Medium	Other medium: Base medium: DMEM/F-12 Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit bFGF 2-mercaptoethanol

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
HESCA-1	Yes

Microbiology / Virus Screening
HIV 1	Negative
HIV 2	Negative
Hepatitis B	Negative
Hepatitis C	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 47XY, +21
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications

Down syndrome Synonyms DOWN'S SYNDROME Trisomy 21 NOS Trisomy 21, Mitotic Nondisjunction Trisomy 21, Meiotic Nondisjunction Down's syndrome NOS (disorder) Complete trisomy 21 syndrome (disorder) G Trisomy Down's syndrome NOS Partial Trisomy 21 Down Syndrome Down Syndrome, Partial Trisomy 21 Syndrome, Down's T21 - Trisomy 21 Syndrome, Down trisomy 21 syndrome Mongolism Downs Syndrome Trisomy 21 (Down Syndrome)Downs SyndromeTrisomy 21 Syndrome Down's syndrome - trisomy 21 Downs syndrome trisomy 21 (Down syndrome) Down's syndrome G trisomy Down syndrome Complete trisomy 21 syndrome Down syndrome, Isolated cases leukemia, megakaryoblastic, with or without Down syndrome, somatic Trisomy 21 complete trisomy 21 syndrome trisomy 21 Down syndrome chromosome region Down syndrome critical region leukemia, megakaryoblastic, of Down syndrome transient myeloproliferative disorder of Down syndrome show more synonyms show less synonyms