HPSI0416i-aicq_4

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name WTSIi466-B
Alternative name(s)
HPSI0416i-aicq_4
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 9th May 2018
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA4754668
ECACC 77650549
HipSci HPSI0416i-aicq_4
Cellosaurus CVCL_IX56

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity British Asian - Indian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
Synonyms
  • Obstructive hypertrophic cardiomyopathy
  • Hypertrophic subaortic stenosis

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4448501

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell origin
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
Age of donor (at collection) 55-59
Collected in 2016
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
33.764 1.642

Certificate of Analysis

Is there a certificate of analysis available?
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0416i-aicq_4
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 0
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003523
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003523
Raw sequencing reads
RNA-seq
https://ega-archive.org/datasets/EGAD00001003540
Abundances of transcripts
RNA-seq
https://ega-archive.org/datasets/EGAD00001003540
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003523
BWA alignment