HPSI0416i-ourj_2

General#

Cell Line

hPSCreg Name WTSIi483-B
Alternative name(s)
HPSI0416i-ourj_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 14th May 2018
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Provider

Generator Wellcome Trust Sanger Institute (WTSI)
Distributors

External Databases

BioSamples SAMEA4762168
ECACC 77650508
HipSci HPSI0416i-ourj_2
Cellosaurus CVCL_IX62

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex male
Ethnicity British Asian - Bangladeshi

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
Synonyms
  • Obstructive hypertrophic cardiomyopathy
  • Hypertrophic subaortic stenosis

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4448503

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell origin
blood
A fluid that is composed of blood plasma and erythrocytes.
Synonyms
  • portion of blood
  • vertebrate blood
Age of donor (at collection) 25-29
Collected in 2016
Source cell line vendor University College London

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Notes on reprogramming vector detection CytoTune 2

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Pluripotency Score Novelty Score
18.772 1.708

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0416i-ourj_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 1
RNA-seq
https://ega-archive.org/datasets/EGAD00001003540
Splice-aware STAR alignment
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003523
Raw sequencing reads
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003523
mpileup variant calls
Exome sequencing
https://ega-archive.org/datasets/EGAD00001003523
BWA alignment
RNA-seq
https://ega-archive.org/datasets/EGAD00001003540
Abundances of transcripts