AD]FiPSAG07645-4F-17

General#

Cell Line

hPSCreg Name ESi035-A
Alternative name(s)
AD]FiPSAG07645-4F-17
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Spanish Stem Cell Bank (ES)
Owner Centre of Regenerative Medicine in Barcelona - Banc de Linies Cellulars (CRMB)
Distributors
Derivation country Spain

External Databases

Cellosaurus CVCL_V152

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Alzheimer's disease
The donor is affected.
Synonyms
  • Disease, Alzheimer
  • Dementia in Alzheimer's disease, unspecified (disorder)
  • Presenile Alzheimer Dementia
  • ALZHEIMERS DIS
  • Alzheimers
  • DAT - Dementia Alzheimer's type
  • Dementia in Alzheimer's disease
  • Alzheimer's disease, NOS
  • Alzheimer Dementia, Presenile
  • Dementia, Alzheimer Type
  • Alzheimer Dementia
  • Alzheimer's Dementia
  • Alzheimer's
  • Dementia, Presenile
  • [X]Dementia in Alzheimer's disease (disorder)
  • ALZHEIMER DIS
  • AD
  • [X]Dementia in Alzheimer's disease
  • AD - Alzheimer's disease
  • Disease, Alzheimer's
  • Alzheimer Disease
  • Dementia, Presenile Alzheimer
  • Alzheimer Type Dementia
  • Dementia in Alzheimer's disease (disorder)
  • Alzheimer's disease (disorder)
  • Alzheimers Dementia
  • Dementia of the Alzheimer's type
  • sporadic Alzheimer's disease
  • Alzheimers disease
show more synonyms

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell line name AG07645
Other names
Source cell type
AG07645 cell
Collected in 2009
Source cell line vendor Coriell

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Selection criteria for clones the clones have been selecte by their morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells CCD1112Sk
Cellfinder Ont Id: CELDA_000001419
Passage method Mechanically
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: K-Dmem
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
GlutaMAX 2mmol/l %
2-mercaptoetanol 0,05mmol/l
bFGF 8ng/ml
NEAA 1%

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
ASA
Yes
ASMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
GFAP
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)