iWSM-F1

General

Cell Line

hPSCreg Name
HUJIi001-A
Alternative name(s)
iWSM-F1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th April 2020
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Provider

Generator Hebrew University of Jerusalem (HUJI)
Derivation country Israel

External Databases

BioSamples SAMEA6907397
CLO CLO_0102696

General Information

Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity Yemenite Jews

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
developmental and epileptic encephalopathy, 28
The donor is a carrier of a mutation in the WWOX gene that is associated with WWOX-Related Epileptic Encephalopathy (WOREE syndrome).
The donor is a carrier of a disease-associated mutation and not affected.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Donor Relations

All cell lines of this donor's relatives
Has son:

External Databases (Donor)

BioSamples SAMEA6914456

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink Dr. Ehud Banne [Kaplan Medical Center]
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Kaplan Medical Center
Approval number 139073-0126-18-KMC
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Kaplan Medical Center
Approval number 139073-0126-18-KMC
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral blood mononuclear cell
Synonyms
  • Peripheral blood monocyte

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Gelatin
Feeder cells Mouse embryonic fibroblast cell
Cellfinder Ont Id: http://cellfinder.org/ontology?id=CF_43885
Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SOX2
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)