General

Cell Line
hPSCreg name	ICGi060-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	ICGi060-A
Alternative name(s)	PD92-22
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	26th May 2025
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Provider
Generator	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG) Contact: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors	Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Derivation country	Russia
General Information
* Is the cell line readily obtainable for third parties?	Yes Research use: allowed Clinical use: not allowed Commercial use: not allowed

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Parkinson Disease The donor is a carrier of a disease-associated mutation and affected. Synonyms Parkinson Disease Parkinson's disease Parkinson's Disease Genetic variants PRKN (target) Chromosome location 6q26 Nucleotide sequence variant in HGVS format NM_004562.3: c.101_102del Protein sequence variant in HGVS format NP_004553.2:p.Gln34fs Is the variant homozygous or heterozygous? Heterozygous ClinVar VCV000425403.51
Karyotyping (Donor)
Has the donor karyotype been analysed?	No
Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?	No

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Alternatives to consent are available?	No
Is there other documentation provided to the donor for consenting purposes?	No
Confirm that consent was obtained by a qualified professional	Yes
Has the donor agreed to be re-contacted?	Yes
Has the donor been informed about how her/his data will be protected?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
* Does consent expressly prevent the derivation of pluripotent stem cells?	No
* Does consent pertain to a specific research project?	No
Does consent permit unforeseen future research, without further consent?	Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications?	No
Does consent expressly prevent development of commercial products?	Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it?	Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time?	Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time?	Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	No
Does consent permit research by
an academic institution?	Yes
a public organisation?	No
a non-profit company?	No
a for-profit corporation?	No
Does consent expressly permit collection of genetic information?	Yes
Does consent expressly permit storage of genetic information?	Yes
Does consent prevent dissemination of genetic information?	Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens?	Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor?	Yes
How may genetic information associated with the cell line be accessed?	Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells?	Yes
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples?	Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor?	Yes
Does consent permit access to medical records of the donor?	No
Does consent permit access to any other source of information about the clinical treatment or health of the donor?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	BUDGETARY INSTITUTION OF HIGHER EDUCATION OF KHANTY-MANSIYSK AUTONOMOUS OKRUG-YUGRA Surgut State University, Medical Institute
Approval number	2
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells?	Yes
Name of accrediting authority involved?	SIYSK AUTONOMOUS OKRUG-YUGRA Surgut State University, Medical Institute
Approval number	2
Do you have obligations to third parties in regard to the use of the cell line?	No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells?	No
Is there an MTA available for the cell line?	No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?	Addgene
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above?	No

hIPSC Derivation

General
Source cell type	Mononuclear cell A cell having only one nucleus, especially: MONOCYTE.
Source cell origin	Blood A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood. Synonyms Reticuloendothelial System, Blood Peripheral Blood peripheral blood blood Blood Whole Blood BLOOD portion of blood vertebrate blood show more synonyms show less synonyms
Collected in	2024
Passage number reprogrammed	1
Reprogramming method
Vector type	Non-integrating
Vector	Episomal
Genes	POU5F1 SOX2 KLF4 MYC mp53DD LIN28
Is reprogramming vector detectable?	No
Methods used	PCR
Files and images showing reprogramming vector expressed or silenced	2025-05-24_13-03-09.png
Vector free reprogramming
Other
Selection criteria for clones	The selection of colonies was carried out according to morphological criteria. We selected flat monolayer colonies with tightly packed cells with high nuclear/cytoplasmic ratio. Embryonic stem cell-like clones were picked by a glass microcapillary.
Derived under xeno-free conditions	No
Derived under GMP?	No
Available as clinical grade?	No

Culture Conditions

Surface coating	Gelatin
Feeder cells	mouse embryonic fibroblasts
Passage method	Enzymatically TrypLE
O2 Concentration	20 %
CO2 Concentration	5 %
Medium	Other medium: Base medium: Knockout DMEM (Life Technologies) Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements Supplement Amount Unit NEAA 0.1 mM GlutaMAX 2 mM rhFGF basic 10 ng/ml Penicillin 50 U/ml Streptomycin 50 µg/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles

Morphology

Morphology pictures

Morphology of ICGi060-A IPSC.tif

Morphology of ICGi060-A IPSC, the scale bar - 100 mcm

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro spontaneous differentiation

Marker	Expressed
AFP	Yes
CK18	Yes

Morphology

AFP expression (red) and DAPI (blue) and CK expression (green) in ICGi060-A

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro spontaneous differentiation

Marker	Expressed
CD29	Yes
ACTA2	Yes

Morphology

ACTA2 expression (red) and DAPI (blue) and CD29 expression (green) in ICGi060-A

Ectoderm

Ectoderm
Ont Id: UBERON_0000924

In vitro spontaneous differentiation

Marker	Expressed
TUBB3	Yes
Neurofilament 200	Yes

Morphology

TUBB3 expression (red) and DAPI (blue) and Neurofilament 200 expression (green) in ICGi060-A

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes Karyotyping and G-banding show ICGi060-A iPSCs have a normal 46, XY karyotype at passage 21. PD92-22-karyotype_(1).jpg Passage number: 21 Karyotyping method: G-Banding
Other Genotyping (Cell Line)