H9.2, WA09.2

General

Cell Line

hPSCreg name WAe009-A-2
Cite as:
WAe009-A-2 (RRID:CVCL_C812)
Alternative name(s)
H9.2, WA09.2
Cell line type Human embryonic stem cell (hESC)
Similar lines
WAe009-A
(WA09, H9)
WAe009-A-77
(H9-GFP)
WAe009-A-1H
(MYL3-KO)
WAe009-A-1I
(H9::GFP cyto-reporter line)
WAe009-A-1J
(H9::mcherry cyto-reporter line)
WAe009-A-78
(TBX18-KO)
WAe009-A-7
(H9-mHOXA9)
WAe009-A-R
(H9-NRL-GP, WA09 NRL+/eGFP)
WAe009-A-36
(JPH2-KO)
WAe009-A-12
(H9_RB1ex3_C7)
WAe009-A-13
(H9_RB1ex3_G12LS)
WAe009-A-1E
(TBX20-KO)
WAe009-A-37
(H9-GSX2-tTA:GFP)
WAe009-A-99
(dCas9-p300 H9 21)
WAe009-A-1V
(H9-AAVS1-Teton-KrasG12D)
WAe009-A-62
(KCNQ1 KO)
WAe009-A-1G
(ISL1-KO)
WAe009-A-90
(H9_LCCS1)
WAe009-A-72
(COL1A2 -/-)
Last update 22nd May 2019
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Provider

Generator WiCell Research Institute (WA)
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_C812
Wikidata Q54993564

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity N/A

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history NO
Is the medical history available upon request? NO
Is clinical information available? NO

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA7768918

Ethics

Also have a look at the ethics information for the parental line WAe009-A .
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

The source cell information can be found in the parental cell line WAe009-A.

Culture Conditions

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)

Genetic Modification